Journal: Journal of Inflammation (London, England)

Article Title: Extracorporeal immune therapy with immobilized agonistic anti-Fas antibodies leads to transient reduction of circulating neutrophil numbers and limits tissue damage after hemorrhagic shock/resuscitation in a porcine model

doi: 10.1186/1476-9255-7-18

Figure Lengend Snippet: Heme oxygenase-1 (HO-1) gene expression (A), and HO-1 protein expression (B) in control (white bars), SMC (grey bars), and LIM (black bars) animals .

Article Snippet: After three washes in TBS containing 0.1% Tween-20, the membranes were incubated for 60 min at room temperature with the horseradish peroxidase-labelled polyclonal goat anti-mouse secondary antibody for HO-1 (Dako Cytomation, Glostrup, Denmark), diluted 1:1,000 in TBS, 0.1% Tween-20 and washed as described above.

Techniques: Expressing

Journal: Redox Biology

Article Title: Dietary intake of deuterium oxide decreases cochlear metabolism and oxidative stress levels in a mouse model of age-related hearing loss

doi: 10.1016/j.redox.2022.102472

Figure Lengend Snippet: Effect of dietary D 2 O supplementation on HO-1 expression in the cochlea of DBA/2J, C57BL/6J and CBA/CaJ mice. A ) Western blot analysis of HO-1 expression in the cochlea of mice at 9 weeks of age; DG, D 2 O administration group; CG, control group. B ) Histograms (mean ± SEM) of blot optical density values normalized to actin. C ) Immunofluorescence analysis of HO-1 expression (green fluorescence) in the organ of Corti (Co), spiral ganglion neurons (SGNs) and stria vascularis (SV) at 9 weeks of age in DBA/2J DG and CG mice. Scale bars: 200 μm; Co, 20 μm; SGNs, 15 μm; StV, 50 μm. D ) Histograms (mean ± SEM) of fluorescence intensity values for HO-1. Experiments were performed in triplicate, and p-values (P) in (B) and (D) were determined by two-tailed t -test. *, P<0.05; **, P<0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: After washing in 0.01 M PBS for 15 min three times, samples were incubated in fluorescently labeled secondary antibodies: goat anti-rabbit (HO-1) (Alexa Fluor 488 # A-11034, ThermoFischer Scientific) or donkey anti-mouse (GSH, Nrf2) (Alexa Fluor 594, #ab150108, Abcam, USA) diluted 1:500 in 0.01 M PBS at room temperature for 2 h. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI).

Techniques: Expressing, Western Blot, Immunofluorescence, Fluorescence, Two Tailed Test

Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

Article Title: Effect of the flavonoids quercetin and taxifolin on UVA-induced damage to human primary skin keratinocytes and fibroblasts.

doi: 10.1007/s43630-021-00140-9

Figure Lengend Snippet: Fig. 9 Effect of flavonoids on UVA-affected HO-1 level and expression in NHDF. HO-1 protein level was analysed by Western blot (A) and HO-1 mRNA level by qPCR (B). Fibroblasts pre-treated with TA and QE (1 h) were non- irradiated (− UVA) or exposed to 7.5 J/cm2 (+ UVA). After incubation (24 h), HO-1 level was evaluated as described in Materials and Methods. Data are normalized to the reference protein actin and gene GAPDH. Results are mean ± SD of 3 experiments employing cells from 3 donors. Representative examples of Western blot analy- sis are shown. *Significantly different from irradiated control cells at p = 0.05. #Significantly different from control cells at p = 0.05

Article Snippet: Western blotting luminol reagent (ImmunoCruzTM) for chemiluminescent horseradish peroxidase (HRP) detection, Nrf2 rabbit polyclonal IgG (C-20, sc-722), heme oxygenase 1 (HO-1) rabbit polyclonal IgG (H-105, sc-10789), NQO1 goat polyclonal IgG (R-20, sc-16463), catalase (CAT) rabbit polyclonal IgG (sc-50508), caspase-3 rabbit polyclonal IgG (for detection of pro-caspase-3 and caspase-3), actin goat polyclonal IgG (I-19, sc-1616), goat anti-rabbit and rabbit antigoat HRP-conjugated secondary antibody were purchased from Santa Cruz Biotechnology Inc. (USA).

Techniques: Expressing, Western Blot, Irradiation, Incubation, Control

Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

Article Title: Effect of the flavonoids quercetin and taxifolin on UVA-induced damage to human primary skin keratinocytes and fibroblasts.

doi: 10.1007/s43630-021-00140-9

Figure Lengend Snippet: Fig. 10 Effect of flavonoids on UVA-affected HO-1 level and expression in NHEK. HO-1 protein level was analysed by Western blot (A) and HO-1 mRNA level by qPCR (B). NHEK pre-treated with TA and QE (1 h) were non-irradiated (− UVA) or exposed to 10 J/ cm2 (+ UVA). After incubation (6 h), HO-1 level was evaluated as described in Materials and Methods. Data are normalized to the reference protein actin and gene GAPDH. Results are mean ± SD of 3 experiments employing cells from 3 donors. Representative examples of Western blot analysis are shown. *Significantly different from irradiated control cells at p = 0.05. #Significantly different from control cells at p = 0.05

Article Snippet: Western blotting luminol reagent (ImmunoCruzTM) for chemiluminescent horseradish peroxidase (HRP) detection, Nrf2 rabbit polyclonal IgG (C-20, sc-722), heme oxygenase 1 (HO-1) rabbit polyclonal IgG (H-105, sc-10789), NQO1 goat polyclonal IgG (R-20, sc-16463), catalase (CAT) rabbit polyclonal IgG (sc-50508), caspase-3 rabbit polyclonal IgG (for detection of pro-caspase-3 and caspase-3), actin goat polyclonal IgG (I-19, sc-1616), goat anti-rabbit and rabbit antigoat HRP-conjugated secondary antibody were purchased from Santa Cruz Biotechnology Inc. (USA).

Techniques: Expressing, Western Blot, Irradiation, Incubation, Control

Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

Article Title: Effect of the flavonoids quercetin and taxifolin on UVA-induced damage to human primary skin keratinocytes and fibroblasts.

doi: 10.1007/s43630-021-00140-9

Figure Lengend Snippet: Fig. 11 Effect of flavonoids on UVA-affected NQO1 level and expression in NHDF. NQO1 protein level was analysed by Western blot (A) and NQO1 mRNA level by qPCR (B). Fibroblasts pre-treated with TA and QE (1 h) were non- irradiated (− UVA) or exposed to 7.5 J/cm2 (+ UVA). After incubation (24 h), HO-1 level was evaluated as described in Materials and Methods. Data are normalized to the reference protein actin and gene GAPDH. Results are mean ± SD of 3 experiments employing cells from 3 donors. Representative examples of Western blot analy- sis are shown. *Significantly different from irradiated control cells at p = 0.05. #Significantly different from control cells at p = 0.05

Article Snippet: Western blotting luminol reagent (ImmunoCruzTM) for chemiluminescent horseradish peroxidase (HRP) detection, Nrf2 rabbit polyclonal IgG (C-20, sc-722), heme oxygenase 1 (HO-1) rabbit polyclonal IgG (H-105, sc-10789), NQO1 goat polyclonal IgG (R-20, sc-16463), catalase (CAT) rabbit polyclonal IgG (sc-50508), caspase-3 rabbit polyclonal IgG (for detection of pro-caspase-3 and caspase-3), actin goat polyclonal IgG (I-19, sc-1616), goat anti-rabbit and rabbit antigoat HRP-conjugated secondary antibody were purchased from Santa Cruz Biotechnology Inc. (USA).

Techniques: Expressing, Western Blot, Irradiation, Incubation, Control

Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

Article Title: Effect of the flavonoids quercetin and taxifolin on UVA-induced damage to human primary skin keratinocytes and fibroblasts.

doi: 10.1007/s43630-021-00140-9

Figure Lengend Snippet: Fig. 12 Effect of flavonoids on UVA-affected NQO1 level and expression NHEK. NQO1 protein level was analysed by Western blot (A) and NQO1 mRNA level by qPCR (B). Keratinocytes pre-treated with TA and QE (1 h) were non- irradiated (− UVA) or exposed to 10 J/cm2 (+ UVA). After incubation (24 h), HO-1 level was evaluated as described in Materials and Methods. Data are normalized to the reference protein actin and gene GAPDH. Results are mean ± SD of 3 experiments employing cells from 3 donors. Representative examples of Western blot analy- sis are shown. *Significantly different from irradiated control cells at p = 0.05

Article Snippet: Western blotting luminol reagent (ImmunoCruzTM) for chemiluminescent horseradish peroxidase (HRP) detection, Nrf2 rabbit polyclonal IgG (C-20, sc-722), heme oxygenase 1 (HO-1) rabbit polyclonal IgG (H-105, sc-10789), NQO1 goat polyclonal IgG (R-20, sc-16463), catalase (CAT) rabbit polyclonal IgG (sc-50508), caspase-3 rabbit polyclonal IgG (for detection of pro-caspase-3 and caspase-3), actin goat polyclonal IgG (I-19, sc-1616), goat anti-rabbit and rabbit antigoat HRP-conjugated secondary antibody were purchased from Santa Cruz Biotechnology Inc. (USA).

Techniques: Expressing, Western Blot, Irradiation, Incubation, Control

Journal: Scientific Reports

Article Title: Forensic significance of intracardiac heme oxygenase-1 expression in acute myocardial ischemia

doi: 10.1038/s41598-021-01102-y

Figure Lengend Snippet: The expression of HO-1 on the human hearts. ( a ) Immunohistochemical analysis by using anti-HO-1. Representative results from the hearts of non-AIHD (Drowning) and AIHD groups were shown here. ( b ) Percentage of cases where HO-1-positive nuclei of cardiomyocytes were exist.

Article Snippet: The following polyclonal or monoclonal Abs (pAbs or mAbs) were used for immunohistochemical analyses in the present study: goat anti-human HO-1 pAbs (1:200, #ADI-SPA-896, Enzo Life Science, Farmingdale, NY), rabbit anti-myeloperoxidase (MPO) pAbs (1:100, RB-373-A, Lab Vision/Neo Markers, Fremont, CA), mouse anti-human macrophage marker (CD68) mAb (1:100, clone MAC387, sc-66204, Santa Cruz, Dallas, TX), rabbit anti-human myoglobin pAbs (1:2000, #A0324, DAKO, Santa Clara, CA).

Techniques: Expressing, Immunohistochemical staining